Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TBS Buffer, Low Salt (10X), 1 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Bioworld TE (Tris-EDTA) Buffer, Sterile, 100 mL
Product DescriptionTE (Tris-EDTA) is a sterile buffer that has a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse activity is detected.TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Bioworld TBE Buffer (25X), 4 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 25X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 25L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Gold Biotechnology Inc MES Free Acid Monohydrate
MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Bioworld TE Buffer (10X), 500 mL
Product DescriptionTE (Tris-EDTA) has a liquid concentrate of 10X with a pH level of 8.0 brought up with HCl and is stored at room temperature. It contains 10 mM Tris and 1 mM EDTA. It is of biotechnology grade and no DNAse or RNAse activity is detected. TE buffer is used to solubilize DNA or RNA while protecting it from degradation. Commonly used for DNA isolation and electrophoresis protocols.
Bioworld TBE Sample Buffer (6X), 50 mL
Product DescriptionTBE (Tris-Borate-EDTA) is a sample buffer used for nucleic acid analysis. It has a 6X concentration, 270 mM Tris base, 270 mM boric acid, 40% glycerol, 0.025% bromophenol blue G, 0.025% xylene cyanol, and 6 mM of EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld Tris-Glycine (TG) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine (TG) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris base and 0.192M Glycine. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated using bioPlus Tris powder to ensure exceptional purity along with the absence of nuclease and protease activities. TG Buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Bioworld TBE Sample Buffer (6X), 25 mL
Product DescriptionTBE (Tris-Borate-EDTA) is a sample buffer used for nucleic acid analysis. It has a 6X concentration, 270 mM Tris base, 270 mM boric acid, 40% glycerol, 0.025% bromophenol blue G, 0.025% xylene cyanol, and 6 mM of EDTA.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-250.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 250 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Teknova Sterile 0.9% Saline Solution. 500 ml.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Teknova DNA Suspension Buffer, pH 8.0. DNase/RNase Tested, PCR Grade. 1mL Tubes, 50-Pack, Sterile.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Alkali Scientific CellPro™ PBS 1X, pH 7.4, with Sodium Azide, FAcs Washing Grade, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ Tris-HCl Buffer, 1M, pH 7.4, 1L, 12/cs
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Research Products International Corp PIPES Sodium Salt 250 G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
